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NASBA


NASBA PRODUCTS

Catalog Number Description Composition Per Reaction Price Per Vial
LEM-5 Lyophilized Enzymes Mix for
RNA Amplification (NASBA)
  • AMV RT 6.4 units
  • RNase H 0.08 units
  • T7 RNAP 32 units
  • High molecular weight sugar mix
  • $ 24.00 each
  • Call for Bulk pricing
This is a lyophilized enzyme mix containing a three-enzyme mixture used in isothermal RNA Amplification reactions. In this reaction, the major product is an anti-sense, single-stranded RNA. This three-enzyme mixture consists of Avian Myeloblastosis Virus Reverse Transcriptase, RNase H and T7 RNA Polymerase. One vial is enough for five (5) reactions at 5 µL per reaction.
Catalog Number Description Composition Per Reaction Price Per Vial
LRB-5 Lyophilized Reaction Buffer for
RNA amplification (NASBA)
  • KCl 140 mM; MgCl2 24 mM;
  • DTT 10 mM;
  • ATP 4.0 mM; CTP 4.0 mM;
  • GTP 3.0mM; UTP 4.0 mM;
  • ITP 1.0 mM;
  • dATP 2.0 mM; dCTP 2.0 mM;
  • dGTP 2.0 mM; dTTP 2.0 mM
  • $ 22.50 each
  • Call for Bulk pricing
LRB-5 is a lyophilized concentrated buffer containing the stock reagents for use in RNA Amplification (NASBA) reactions. When reconstituted with Reconstitution buffer DRB-5, it is a 2X buffer. One vial is enough for 5 reactions at 10 µL per 20 µL reaction.
Catalog Number Description Composition Per Reaction Price Per Vial
DRB-5 Reaction Reconstitution Buffer
for LRB-5(60 uL)
  • Tris Base 80 mM
  • DMSO 30 %
  • pH 8.5 at RT
  • $ 5.00 each
Catalog Number Description Composition Per Reaction Price Per Vial
D-PDG-5 PDGT diluent for LEM-5(40 uL)
  • 10 mM Potassium Phosphate Buffer
  • 2 mM Dithiothreitol
  • 10% (v/v) Glycerol
  • 2% (v/v) Triton X-100
  • pH 7.2
  • $ 5.00 each


NASBA ENZYME COCKTAIL

Catalog Number Description Composition Per Reaction Price Per Vial
NEC-1-24 NASBA Enzyme Cocktail
(wet mix)(120 uL)
  • AMV RT 6.4 units
  • RNase H 0.08 units
  • T7 RNAP 32 units
  • BSA 0.48 mg/ml 2.4 µg
  • High MW sugar matrix
  • $ 84.50 each
  • Call for Bulk pricing
The Enzyme Cocktail is used in Nucleic Acid Sequence Based Amplifications. NASBA is an isothermal method producing antisense, single-stranded RNA as the major amplification product. This enzyme cocktail is a mixture, consisting of Avian Myeloblastosis Virus Reverse Transcriptase, RNase H and T7 RNA Polymerase. One vial is enough for 24 reactions at 5 µL per reaction.


NASBA ENZYME COMPANION PRODUCTS

Catalog Number Description Composition Per Reaction Price Per Vial
NECB-24 3X NASBA Reaction Buffer
for NEC-1-24 (200 µL)
  • 40 mM TrisHCl, pH 8.5 @ 25ºC
  • 12 mM MgCl2
  • 70 mM KCl
  • 10 mM DTT
  • 15% Dimethyl Sulfoxide
  • $ 5.00 each
  • Call for Bulk pricing
This reaction buffer is to be used in conjunction with the liquid NASBA Enzyme Cocktail (NEC-1-24) in NASBA reactions. It requires the addition of nucleotides by the user.
NECN-24 6X Nucleotide Mix
for NEC-1-24 (84 µL)
  • dATP – 6.0 mM
  • dCTP – 6.0 mM
  • dGTP – 6.0 mM
  • dTTP – 6.0 mM
  • ATP – 12.0 mM
  • CTP – 12.0 mM
  • GTP – 9.0 mM
  • UTP – 12.0 mM
  • ITP – 3.0 mM
  • $ 75.00 each
  • Call for Bulk pricing
This reaction buffer is to be used in conjunction with the liquid NASBA Enzyme Cocktail (NEC-1-24) and 3X Reaction Buffer (NECB-24) to supply necessary nucleotides in NASBA reactions.





Real-time detection of RNA Product Using a Molecular Beacon