NASBA
NASBA (Nucleic acid sequence-based amplification) is an isothermal nucleic acid amplification method suited to detection and quantification of genomic, ribosomal or messenger RNA. NASBA reactions produce antisense, single-stranded RNA as the major product.  Potential RNA analytes identified by NASBA include viral load, viable retroviruses, replicating bacteria and disease states such as cancer.

  • Forward and reverse primers which include T7 promoter sequences are required
  • Real time quantitative detection is accomplished fluorimetrically by the inclusion of specific molecular beacon reporter probes
  • Reactions are conducted isothermally at 41ºC, without the aid of thermocyclers.

Lifes Sciences Advanced Technologies provides both liquid and lyophilized reagents for NASBA.
See list of lyophilized reagents

See liquid reagents