T4 RNA Polymerase

• Isolated from E. coli transformed with T7 bacteriophage
• Recognizes and initiates synthesis of RNA from double-stranded DNA templates that carry the T7-specific promoter
• Suitable for use in performance of NASBA amplification of RNA substrates in conjunction with AMV RT and RNase H
• Tested for RNase and DNase activity
• Bulk quantities available

GT7 005 is the enzyme prepared in 20 mM KPi, pH 7.5, mM NaCl, 1.0 mM EDTA, 2.0 mM DTT, 0.2% Triton X-100 and 50% glycerol.

Concentration: 50 U/µL
Storage: Store at -20oC