AMV Reverse Transcriptase, 95%
  • Isolated from avian myeloblastosis virus
  • Synthesis of cDNA using RNA, DNA or RNA-DNA hybrids as templates
  • Provides excellent performance in first-strand and second-strand cDNA synthesis for RT-PCR, cloning, dideoxy sequencing, and NASBA
  • Performs at higher reaction temperatures (37-50°C)
  • Intrinsic RNase H activity
  • 95% Pure by SDS-PAGE
  • Tested for RNase and DNase activity
  • Bulk quantities available

Presentations
AMV 007 is reverse transcriptase in 0.2 M potassium phosphate buffer, pH 7.2; 0.2 % Triton X-100; 2.0 mM DTT; 50% glycerol Concentration: 20 U/µL
Shipped with: 10X/5X Reaction Buffer, Protocols for use in cDNA synthesis
Storage: Store at -70oC or -20oC
AMV 005 is reverse transcriptase in lower concentration enzyme in 0.2 M potassium phosphate buffer, pH 7.2; 0.2 % Triton X-100; 2.0 mM DTT; 50% glycerol. Compare to Promega, NE Biolabs. Concentration: 10 U/µL
Shipped with: 10X/5X Reaction Buffer, Protocols for use in cDNA synthesis
Storage: Store at -70oC or -20oC
RTHSA35 (prev. LME704)is a higher specific activity form of AMV reverse transcriptase. Functionally tested for use in cDNA synthesis without RNase inhibitor.  Particularly suited for longer RNA templates.  In 0.2 M potassium phosphate buffer, pH 7.2; 0.2 % Triton X-100; 2.0 mM DTT; 50% glycerol Concentration: 35 U/µL
Shipped with: 10X/5X Reaction Buffer, Protocols for use in cDNA synthesis
Storage: Store at -70 oC or -20oC
AMVT43 is AMV-RT in trehalose for lyophilization.  In 0.2 M KPi, pH 7.2; 0.2 % Triton X-100; 4.0 mM DTT; 1.0 M trehalose.Concentration: 15 U/µL
Shipped with: 10X/5X Reaction Buffer, Protocols for use in cDNA synthesis
Storage: Store at -70 oC
ARB 45 5X/10X Reaction Buffer is available free,  in appropriate quantity, with any order of AMV RT.  Functionally tested in a cDNA synthesis reaction.  Can be used either as a 10X or a 5X stock.  Composition: 250 mM  Tris-HCl, pH 8.3, 500 mM KCl, 20 mM DTT, 50 mM  MgCl2.Storage: Store at -70oC or -20oC
Unit Definition:
The amount of enzyme required to catalyze the incorporation of 1 nanomole of thymidine triphosphate into acid-precipitable material in 10 minutes at 37ºC using the following reaction conditions:  50 mM TrisHCl @pH 8.3, 40 mM KCl, 6mM MgCl2 ,  4.0 mM Dithiothreitol, 0.4 mM  rAn.dT12-18, and 0.5 mM tritiated Thymidine triphosphate.
Code No. Description Quantity Price
AMV 007-1 AMV-RT at 20 U/μL 1000 U $ 200.00
AMV 007-5 AMV-RT at 20 U/μL 5000 U $ 850.00
AMV 007-7 AMV-RT at 20 U/μL ≥ 50,000 U Inquire
AMV 005-1 AMV-RT at 10 U/μL 500 U $ 100.00
AMV 005-2 AMV-RT at 10 U/μL 1000 U $ 170.00
NEW Size AMV 005-3 AMV-RT at 10 U/μL 300 u $ 66300
NEW AMVT43-1 AMV-RT at 15 U/μL in trehalose 1000 U Inquire
NEW AMVT43-5 AMV-RT at 15 U/μL in trehalose 5000 U
RTHSA35-1 AMV-RT (HSA) at 35 U/μL (LME704) 500 U $ 155.00
NEW Size RTHSA35-5 AMV-RT (HSA) at 35 U/μL (LME704) 5000 U $ 1200.00