Nucleic Acid Modifying Enzymes

AMV Reverse Transcriptase

  • Isolated from avian myeloblastosis virus originally produced in our facility for the NIH.
  • Native AMV RT produces high molecular weight cDNA.
  • Life Sciences Advanced Technologies is the major source of the native form of this enzyme.

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T7 RNA Polymerase

  • Isolated from E. coli transformed with T7 bacteriophage.
  • Recognizes and initiates synthesis of RNA from double-stranded DNA templates that carry the T7-specific promoter
  • Suitable for use in performance of NASBA amplification of RNA substrates in conjunction with AMV RT and RNase H

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RNase H

  • An endonuclease that specifically degrades the RNA of DNA-RNA hybrids, without effecting unhybribized RNA
  • Tested for RNase and DNase activity

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