AMV RT synthesizes cDNA from single stranded RNA. We provide different concentration 20 K U/mL and 35 K U/mL with specific activity 40,000 U/mg and 55,000 U/mg accordingly. AMV RT is qualified for cDNA synthesis and RT-PCR. AMV RT available in glycerol and trehalose storage buffer (suitable for lyophilization).
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T7 RNA Polymerase catalyses the synthesis of RNA in the presence of double-stranded DNA containing a T7 promoter sequence. It is isolated from an E. coli transformed by a plasmid containing the T7 RNA Polymerase gene. This enzyme is provided in glycerol-based storage buffer and also in 1.0 M trehalose instead of glycerol (suitable for lyophilization).
1LSAT offers diagnostic products for Avian Leukosis Virus: Rabbit anti-avian p27 antiserum, IgG fraction and peroxidase conjugate. The avian leukosis viruses have in common with AMV a group-specific protein, namely p27 protein. Isolation of this purified protein is qualified for diagnosis of avian leukosis viruses in ELISA assays. Purified protein p27 from AMV is used as an antigen to raise high-titered polyclonal antibodies in rabbit antisera.
2LEM-5 is a lyophilized enzyme mix containing a three-enzyme mixture used in isothermal RNA Amplification reactions. In this reaction, the major product is an anti-sense, single-stranded RNA. This three-enzyme mixture consists of Avian Myeloblastosis Virus Reverse Transcriptase, RNase H and T7 RNA Polymerase.
3Trehalose is a proven stabilizer that is effective for many proteins, enzymes and cell membranes. LSAT offers 2 grades of trehalose dihydrate powder, Trehalose Dihydrate Molecular Biology Grade , as well as a 1M sterile solution. There is no detectable RNase or DNase activity, and endotoxin concentration meets U.S.P. requirements. An aqueous solution is suitable as a buffer component in many molecular biology applications, including isothermal and temperature-cycled nucleic acid amplification.
4We have over 50,000 different ELISA kits available for the Human, Rat, Mouse, Rabbit, and other species. If you do not see the kit for your application, please contact us.